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. 2021 Oct 21;297(6):101331. doi: 10.1016/j.jbc.2021.101331

Figure 4.

Figure 4

HFD promotes fructose metabolism in the liver and AC261066 attenuates this effect.A, quantitative comparison of transcript levels of the genes involved in fructose uptake and metabolism from the RNA-seq data. The y axes (relative mRNA levels) are differentially expressed gene (DEG) transcript levels (n = 6 per group). B, immunoblotting analysis of the hepatic protein levels of KHK (Cropped images). Each lane is a sample from one mouse. C, quantification of the KHK immunoblotting data. D, the hepatic levels of metabolites involved in fructose metabolism in all experimental groups (n = 4 per group with two repeats). E, the hepatic levels of metabolites involved in oxidative stress in all experimental groups (n = 4 per group with two repeats). F, AML12 cells were stained with Bodipy reagent to label intracellular lipids after various treatments (Experimental procedures) for 48 h. Quantification of the staining with Fiji (ImageJ) representing the integrated density normalized/number of nuclei (NucBlue)/field. Representative areas highlighted in the insets. Scale bar for the images = 100 μm. Scale bar for the insets = 50 μm. HFD+AC261=HFD+AC261066. ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05, compared with the HFD group. AC261, AC261066; Co, vehicle-treated cells; Fr, fructose; Pl, palmitate.