Figure 3.

REST does not alter EAAT1 expression in astrocytes.A–C, H4 astrocytes were cotransfected with the EAAT2 promoter reporter and/or REST expression vectors, followed by luciferase assay (A), quantitative PCR (B), and Western blotting (C). GAPDH and β-actin were used as loading controls of mRNA and protein, respectively. Data are expressed as mean ± SD. The data shown are representative of three independent experiments. EAAT2, excitatory amino acid transporter 20; REST, repressor element 1-silencing transcription factor.