Fig. 9.

Combination therapy synergistically reduces NB spheroid tumor growth. 3D spheroid tumors were treated with either doxorubicin alone or in combination with BX-795 at indicated doses. (A) Representative images of 3D spheroid tumors showing synergistic reduction in tumor mass. (B) Spheroid tumor mass measurements at different days during treatment. (C) Representative images of terminal spheroids at day 7 stained with Calcein AM and Ethidium homodimer III fluorescence dyes. (D) Quantitative measurement of number of live cells in terminal spheroids at day 7. *** p < 0.001, **** p < 0.0001. Additional data in Supplementary Fig. S3.