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. Author manuscript; available in PMC: 2022 Mar 2.
Published in final edited form as: J Am Coll Cardiol. 2021 Mar 2;77(8):1073–1088. doi: 10.1016/j.jacc.2020.12.060

Figure 1. Characterization of mitochondria-rich extracellular vesicles (M-EVs).

Figure 1.

A. Differential ultracentrifugation was employed to isolate M-EVs. B. Representative flow cytometry (FCM) dot plots and histograms of M-EVs and Large vesicle-depleted EVs (Ld-EVs). C. Transmission electron microscopy of D15 M-EVs. D. The protein expression of the electron transport chain (ETC) in D15 M-EVs. E. M-EVs, but not Ld-EVs, produced extracellular ATP (n=4/group). *P<0.0001 by an unpaired t-test. ATP5A=ATP synthase α-subunit; COX II=cytochrome c oxidase subunit-2; MTDR=mitotracker deep red; MTG=mitotracker green; MTT=3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NDUFB8=NADH dehydrogenase-1β subcomplex subunit-8; SDHB=succinate dehydrogenase complex iron sulfur subunit B; SSA=side-scatter-area; UQCRC2=ubiquinol-cytochrome c reductase core protein-2.