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. Author manuscript; available in PMC: 2022 Mar 2.
Published in final edited form as: J Am Coll Cardiol. 2021 Mar 2;77(8):1073–1088. doi: 10.1016/j.jacc.2020.12.060

Figure 4. PGC-1α knockdown compromised the restoration of mitochondrial bioenergetics and biogenesis in the hypoxia-injured iCMs.

Figure 4.

A. Relative PGC-1α and ERRγ mRNA expression, normalized to U6, in Ld-EVs from D15 transduced with pLKO.1 expressing control shRNA (shCTRL) or anti-PGC-1α shRNA (shPGC-1α) (n=3/group). *P<0.0001 by an unpaired t-test. B. PGC-1α knockdown reduced restoration of ATP levels in hypoxia-injured iCMs at 24hous (n=5–8/group). C. Representative immunoblots and quantitative analyses of PGC-1α, ETC complexes and β-actin (n=4–6/group). *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001 by a one-way ANOVA followed by Tukey’s test. Abbreviations as in Figure 1.