Fig. 2.

Neuroprotection by NP10679 reduces infarct volume in the MCAo model of transient ischemia in mice. (A) NP10679 reduces infarct volumes measured 24 hours post-MCAo with an ED₅₀ of 1 mg/kg, i.p. NP10679 was administered 15 minutes prior to initiation of transient MCAo for 60 minutes. In all experiments, the drug doses were randomized and the investigators were blinded from surgical procedure through analysis of stained sections to determine infarct volume. The plot is pooled data across three independent experiments. Data are means ± S.E.M. for n = 9 (0.2 mg/kg), 13 (0.5 mg/kg), 21 (1 mg/kg), 12 (2 mg/kg), 12 (5 mg/kg), 24 (10 mg/kg), and 34 (Veh) mice. **P < 0.01 from vehicle (ANOVA, Dunnett’s). (B and C) Representative images of TTC-stained sections of vehicle (B) and drug-treated brain. (C). A representative image is shown for each dose, with each brain cut into four sections (top to bottom), and both sides of the section are shown (left and right images). The red portion of the image is TTC stain of undamaged tissue. Vehicle (Veh) is the formulation alone as described in Methods.