Fig. 4.

Side effects measured in Rotarod challenge and locomotor activity. (A) Rotarod. Mice were trained on the Rotarod on 2 consecutive days (days 1 and 2), with four trials per day and an intertrial interval of 25 minutes. On day 3, mice were randomized to groups and administered vehicle (open circle), ifenprodil (open triangle, 30 mg/kg), or NP10679 at 2 mg/kg (light gray circle), 5 mg/kg (medium gray circle), or 10 mg/kg (black circle). The mean ± S.E.M. (n = 8) latency to fall for each trial was calculated for each group. *P < 0.01 from vehicle, ANOVA, Dunnett’s on day 3 within trials was determined. (B) Locomotor activity. Mice were habituated for 1 hour in a closed locomotor activity box and then removed and administered either vehicle (Veh, n = 6), MK-801 (MK at 0.3 mg/kg, n = 4) or NP10679 (20 mg/kg, n = 6 mice) by intraperitoneal injection and then placed back in the boxes, and the horizontal locomotor activity was counted for 2 hours. *P < 0.01, from vehicle (ANOVA, Dunnett’s). Total number of light beam breaks during the sample time are reported on the abscissa, which is representative of horizontal movement. Vehicle (Veh) is the formulation alone as described in Methods.