Fig. 7.

NO nitrosylates AAT1 at Cys192. A, Effect of NO on the S-nitrosylation modified site of purified AAT1 protein. B, Primary HASMCs were treated with 100 μM SNP for 2 h or with SNP (100 μM) for 1 h and then DTT (100 μM) for 1 h after transfection with plasmid. The BSA method was used to detect S-nitrosylation modification of AAT1 protein Cys192 in primary HASMCs (n = 10). C, Primary HASMCs were treated with 100 μM of SNP for 2 h after transfection with plasmids, and AAT1-WT and AAT1-C192S activities in primary HASMCs were detected by colorimetric assay (n = 12). **P < 0.01. Data are expressed as mean ± SEM. AAT, aspartate aminotransferase; DTT, dithiothreitol; NO, nitric oxide; SNP, sodium nitroprusside.