Table 2.
Estimated cell and coliform numbers, and relative total class 1 integron (intI1), anthropogenic class 1 integron (aint1), empty anthropogenic class 1 integron (eaint1), class 2 integron (intI2), and class 3 integron (intI3) per bacteria cell.
| Sample | 16S rRNA (copies/mL)a | Coliforms (copies/mL) | intI1 (copies/cell)b | aint1 (copies/cell) | eaint1 (copies/cell) | intI2 (copies/cell) | intI3 (copies/cell) |
|---|---|---|---|---|---|---|---|
| RU | 8.8 ± 0.11 | 0.24 ± 0.064 | 8.6E-5 ± 9.6E-6 | 1.6E-5 ± 1.2E-5 | NDc | ND | 1.4E-6 ± 2.4E-6 |
| SRU | 11.3 ± 0.052 | 0.12 ± 0.006 | 3.5E-4 ± 8.7E-5 | 2.9E-4 ± 6.8E-5 | 2.2E-5 ± 1.2E-6 | ND | 7.7E-6 ± 4.5E-6 |
| HP_A | 10.1 ± 0.053 | 3.2 ± 2.2 | 1.6E-2 ± 2.6E-3 | 7.9E-3 ± 1.3E-3 | 7.9E-4 ± 1.9E-4 | 1.6E-4 ± 2.3E-5 | 6.6E-3 ± 5.8E-4 |
| HP_B | 9.9 ± 0.038 | 5.7 ± 1.7 | 2.0E-2 ± 5.8E-3 | 1.3E-2 ± 3.4E-3 | 1.1E-3 ± 4.4E-4 | 1.3E-4 ± 3.6E-5 | 1.0E-2 ± 3.4E-3 |
| CM | 9.9 ± 0.14 | 2.3 ± 0.82 | 2.4E-3 ± 1.8E-4 | 1.2E-3 ± 1.1E-4 | 1.3E-4 ± 5.6E-5 | 4.6E-5 ± 2.4E-5 | 1.3E-4 ± 6.0E-5 |
| INF | 10.5 ± 0.37 | 0.59 ± 0.20 | 2.1E-3 ± 3.5E-4 | 1.2E-3 ± 2.7E-4 | 1.5E-4 ± 4.6E-5 | 3.6E-5 ± 6.3E-6 | 1.2E-4 ± 2.4E-5 |
| RAS | 11.7 ± 0.092 | 0.048 ± 0.013 | 2.3E-3 ± 1.4E-3 | 1.7E-3 ± 1.1E-3 | 5.4E-4 ± 4.0E-4 | 5.4E-6 ± 9.2E-7 | 7.8E-5 ± 6.8E-5 |
| EFF | 9.4 ± 0.031 | 1.3 ± 0.79 | 2.0E-3 ± 8.0E-4 | 1.4E-3 ± 6.0E-4 | 1.7E-4 ± 1.1E-4 | 1.1E-5 ± 2.7E-6 | 1.0E-4 ± 3.7E-5 |
| RD | 9.7 ± 0.056 | 0.38 ± 0.048 | 3.2E-3 ± 7.7E-4 | 1.9E-3 ± 6.5E-4 | 3.8E-4 ± 1.3E-4 | ND | 2.1E-4 ± 8.2E-5 |
| SRD | 11.6 ± 0.035 | 0.51 ± 0.17 | 1.9E-3 ± 6.3E-6 | 1.4E-3 ± 4.2E-5 | 3.1E-4 ± 1.4E-5 | 2.9E-5 ± 1.4E-5 | 2.7E-5 ± 7.9E-7 |
On the basis of the Ribosomal RNA Database, the average number of 16S rRNA-encoding genes per genome equivalent was estimated as 4.1. 16S rRNA and coliform gene copy concentrations were divided by this value to estimate bacterial cell numbers or coliforms. For sediment samples, the denominator is sediment mass in gms rather than mL.
The normalized copy numbers of integrons per bacterial cell were calculated by dividing the gene copy number of each integron by the estimated number of bacterial genome equivalents.
ND refers to not detected genes in 5 ng/ul DNA.