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. 2021 Nov;379(2):191–202. doi: 10.1124/jpet.121.000840

Fig. 2.

Fig. 2.

The effect of dipeptides His-Tyr, His-His, and Tyr-His on catalytic activity of recombinant rat Nln. (A) Chemical structures of dipeptides His-Tyr (NSC 374121), His-His (NSC 523374), and Tyr-His. (B–D) Representative reaction progress curves of QFS hydrolysis (15 µM) by recombinant rat Nln (0.3 nM) in the presence of different concentrations of His-Tyr, His-His, and Tyr-His. (E) Concentration-dependent effect of all three dipeptides on hydrolysis of QFS under the same experimental conditions (mean ± S.D., n = 4 independent experiments with duplicate samples for each condition). Note that the initial velocity of hydrolysis in the absence of dipeptides corresponds to 100% on the vertical axis (basal activity) and −13 on the horizontal axis. For His-Tyr, A50 = 89.7 µM (95% CI, 74.2–108.6 µM) and Amax = 449% (95% CI, 432%–468%). For His-His, A50 = 136 µM (95% CI, 104–179 µM) and Amax = 463% (95% CI, 436%–492%). For Tyr-His, A50 = 355 µM (95% CI, 179–780 µM) and Amax = 135% (95% CI, 127%–149%).