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. 2021 Nov;379(2):191–202. doi: 10.1124/jpet.121.000840

Fig. 4.

Fig. 4.

The effect of His-Tyr and His-His on catalytic activity of recombinant human peptidases. All panels document concentration-dependent effect of His-Tyr and His-His on hydrolysis of a respective quenched fluorescence substrate (mean ± S.D., n = 4 independent experiments with duplicate samples for each condition): Mca-Pro-Leu-Gly-Pro-D-Lys(DNP)-OH at 15 µM for thimet oligopeptidase (TOP), Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH at 10 µM for angiotensin converting enzyme (ACE) and neprilysin (NEP), and Mca-Ala-Pro-Lys-(Dnp)-OH at 10 µM for angiotensin converting enzyme 2 (ACE2). In all panels, the initial velocity of the hydrolysis in the absence of either compound corresponds to 100% on the vertical axis and −13 on the horizontal axis.