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The effect of His-Tyr and His-His on catalytic efficiency of Nln. Hydrolysis of different concentrations of synthetic substrate (QFS) by recombinant rat Nln (0.3 nM) in the absence or presence of His-Tyr and His-His (40 and 100 µM) is presented (mean ± S.D., n = 4 independent experiments with duplicate samples for each condition; FLU, fluorescence unit).
