TABLE 3.
Calculated Vmax and Km values for experiments presented in Figure 6
Vmax units are in FLU/ng protein per min, Km units are in µM (FLU, fluorescence unit). Data are presented as means together with 95% confidence intervals in parentheses (n = 4 independent experiments with duplicate samples for each condition; *P < 0.05; ***P < 0.001 in comparison with Nln values).
| Vmax | Km | Vmax/Km | Fold Change | |
|---|---|---|---|---|
| Nln | 96.6 (91.3–101.8) | 13.9 (12.0 –15.8) | 6.9 | — |
| Nln + 40µM His-Tyr | 124.2*** (112.2–136.2) | 7.9*** (5.7–10.1) | 15.7 | 2.3 |
| Nln + 100µM His-Tyr | 164.8*** (147.2–182.4) | 6.4*** (4.3–8.5) | 25.8 | 3.7 |
| Nln + 40µM His-His | 112.5* (102.7–122.3) | 8.0*** (6.0–10.0) | 14.0 | 2.0 |
| Nln + 100µM His-His | 148.2*** (133.0–163.3) | 6.1*** (4.1–8.0) | 24.4 | 3.5 |