FIG. 13.

Immunocytochemical examination of cytochrome c release. The localization of cytochrome c was analyzed in noninduced (OFF) and induced (ON) HSP70 (clone 8-17) cells before (control) and after (HS) heat shock (43°C for 60 min followed by 6 h at 37°C). Cytochrome c has a punctate localization in control cells (A and E). Following a heat shock the mitochondrion-localized immunofluorescence pattern is lost and cytochrome c becomes evenly distributed throughout the cytoplasm (C). Cytochrome c remains localized to mitochondria in cells expressing hsp70 (G). Nuclei were stained with DAPI (B, D, F, and H).