Antibody responses
*P < 0.05, **P < 0.01, and ***P < 0.001, all relative to the unvaccinated group (control). #P < 0.05, ##P < 0.01, ###P < 0.001, all compared with the simple vaccination group (ND).
(A) Serum HI antibody titers (Log2) from each group on the 7th, 14th, 21st, and 28th d after vaccination (n = 5). (B) Antigen-specific IgG and IgA titers in serum from different groups chickens on the 7th, 14th, 21st, and 28th d after vaccination (n = 5).