Figure 3.

CD3⁺CD4⁺ and CD3⁺CD8⁺ T cell activation

*P < 0.05, **P < 0.01, and ***P < 0.001, all relative to the unvaccinated group (control). #P < 0.05, ##P < 0.01, ###P < 0.001, all compared with the simple vaccination group (ND).

(A) The percentages of CD3⁺CD4⁺ and CD3⁺CD8⁺ T spleen lymphocytes isolated from chickens treated with 600 mg /kg, 450 mg/kg, and 300 mg/kg of GPS-1 at 14 d after NDV vaccination (n = 5), and the representative flow cytometry plots of CD3⁺CD4⁺ and CD3⁺CD8⁺ T lymphocytes.

(B) The percentages of CD3⁺CD4⁺ and CD3⁺CD8⁺ T cells isolated from peripheral blood lymphocytes collected from chickens treated with 600 mg /kg, 450 mg/kg, and 300 mg/kg of GPS-1 at 14 d after NDV vaccination (n = 5), and the representative flow cytometry plots of CD3⁺CD4⁺ and CD3⁺CD8⁺ T lymphocytes.