Fig. 3.

HIPK2 inhibition protects acute MI. (a and b) HIPK2 inhibitor (PKI1H) reduced the infarct sizes (IS) and Bax/Bcl₂, cleaved Caspase3/Caspase3 in heart of acute MI mice as determined by TTC staining (n=8 per group) and western blotting (n=3 per group); (c and d) HIPK2^–/– mice reduced IS and Bax/Bcl₂, cleaved Caspase3/Caspase3 in heart of acute MI mice as determined by TTC staining (n=8 in WT+AMI, 7 in HIPK2^–/–+AMI) and western blotting (n=3 per group). Data are represented as mean ± SD. Significant differences were assessed by two-tailed student t-test in a and c, or two-way ANOVA followed by Bonferroni's multiple comparisons test in b and d. *: p<0.05, ***: p<0.001 versus respective control.