FIG. 7.

Vav2 expression elevates the activity of Rac1, Cdc42, and RhoA in cells. (a to c) Blots with anti-Rac1, Cdc42, and RhoA are shown, respectively. In each case, the top panels show immunoblots of the protein that was sedimented by either the GST-PBD beads (i.e., Rac1-GTP or Cdc42-GTP) or the GST-RBD beads (i.e., RhoA-GTP) after incubation with lysates from cells transfected with GFP or Δ184N Vav2. The bottom panels show blots of the corresponding cell lysates. (d) Graphical representation of relative activity of RhoA, Rac1, and Cdc42 in cells transfected with Δ184N Vav2. The activity level of RhoA, Rac1, and Cdc42 in cells transfected with GFP was set at 1. Each bar represents the mean of three separate experiments (error bars, standard error of the mean). For the increase in RhoA and Rac activity, P < 0.001. For the increase in Cdc42 activity, P = 0.03.