Fig. 1.

Affected vs unaffected. Four proteins (NEFM, AQP4, NPTX2 and VGF) selected by both Random forest and LASSO when comparing affected and unaffected individuals. Yellow and circles = affected individuals (n = 39), blue and triangles = unaffected individuals (n = 174). A Violin plots for NEFM, AQP4, NPRX2 and VGF, with p-values from Wilcoxon rank sum test. B A PCA plot based on the four selected proteins. C A hierarchical clustering based on PC1 and PC2. NEFM, neurofilament medium polypeptide; AQP4, apolipoprotein E isoform 4; NPTX2, neuronal pentraxin 2; VGF, neurosecretory protein VGF; AU, arbitrary units; PCA, principal component analysis