Table 2.
Characteristics of patients with HPV positive breast cancers in PCR nested and qPCR genotyping (Amoy test)
| Feature | Sample code | |
|---|---|---|
| 283,618 | 561,482 | |
| Clinical and histopathological features | ||
| Age | 46 | 56 |
| Clinical stage | T2N1 | T2N2 |
| Immunophenotype | Lum B HER2+ | Lum B HER2+ |
| Microscopic characteristics | tightly packed nests of neoplastic cells, with central necrosis, fibrosis and calcification | loosely packed nests of neoplastic cells with areas of necrosis |
| Grade | 3 tubule formation 3 points + nuclear pleomorphism 3 points + mitotic count 3 points | 3 tubule formation 3 points + nuclear pleomorphism 3 Points + mitotic count 2 points |
| Tumour margin | Infiltrative | Infiltrative |
| Tumour infiltrating lymphocytes | < 5% | About 10% |
| Lympho-vascular involvement | Absent | Absent |
| DNA extraction | ||
| Total DNA concentration ± SE [µg] | 5.43 ± 0.61 | 4.30 ± 0.32 |
| A260/280 | 1.74 ± 0.10 | 1.91 ± 0.11 |
| A260/230 | 1.94 ± 0.10 | 1.99 ± 0.14 |
| Amplification of β-actin Ct ± SE | 29.8 ± 0.4 | 29.2 ± 0.1 |
| Nested PCR | ++ | ++ |
| HPV genotyping by qPCR | HPV16 | HPV16 |
| P16 immunostaining | Positive | Positive |
PCR quantitative polymerase chain reaction