Fig. 4.

A, B Purity of γδ T cells during vitro expansion. γδ T cells were treated with Que at 0 µM, 2.5 µM, 5 µM and 10 µM for 10 day or 12 day, respectively. Detecting the frequency of the total lymphocyte cells in CD3 + γδ T cells, Vδ1 + γδ, and Vδ2 + γδ T cell populations. C Cytotoxicity of γδ T cells against target cells at 1:1, 5:1 and 10:1 effector to target ratios detected by CFSE staining methods. D the effector cell: target cell (E/T) was 1:1, 5:1, 10:1 were treated with Que at 5 µM and for 4 h, synergistic cytotoxic effects on breast cancer cells detected by CFSE staining methods. 20,000 cells were analyzed by flow cytometry