FIG. 3.

Analysis of hybrid joints in s/s and s/+ ts-Ab-MLV transformants. (A) Diagram illustrating the formation and the detection of CJ and HJ products. The λ gene segments (rectangular boxes) are flanked by RSS (triangles). Upon recombination cleavage, the Vλ1 coding ends can be resolved into Vλ1Jλ1 CJ or joined to the Jλ1 RSS as HJ (large arrows). Small arrows represent PCR primers used to amplify these joining products. (B) Semiquantitative analysis of (HJ). DNA samples were prepared from both s/s-ts and s/+-ts cells under similar culture conditions, as described in the Fig. 1 legend. (C) Comparison of HJ and intralocus CJ between s/+-ts (s/+) and s/s-ts (s/s) cells. DNA samples were prepared from cells cultured at 33°C (33), at 39°C for 2 days [39(2)], at 39°C for 3 days [39(3)], or at 39°C for 3 days followed by 2 days at 33°C (39-33).