TABLE 1.

Quantitation of relative levels of intralocus and interlocus joining products

Expt	Culture temp conditionsa	Intensity (cpm for sample/cpm for actin)b for:
		s/+ cells			s/s cells
		CJ	IJ	IJ/CJc	CJ	IJ	IJ/CJ
1	33	0.3	<0.01	0.02	<0.01	0.003	—d
	39	6.8	<0.01	<0.01	<0.01	0.004	—
	39–33	6.6	<0.01	<0.01	1.3	0.1	0.08
2	33	1.4	0.02	0.01	<0.01	<0.01	—
	39	3.5	0.02	<0.01	0.14	0.01	0.07
	39–33	3.9	0.02	<0.01	2.8	0.7	0.25
3e	39–33	2.9 ± 0.7	<0.01f	<0.01	2.5 ± 0.2	0.89 ± 0.21f	0.36
			<0.01g	<0.01		0.22 ± 0.05g	0.09

^aCells were cultured at 33°C (33), 39°C for 2 days (39), or 2 days at 39°C followed by an incubation at 33°C for 1 (experiments 1 and 2) or 2 days (experiment 3) (39-33). 

^bValues shown correspond to the amount of joining products relative to the amount for control actin. The intensity of hybridizing bands was quantitated with a PhosphorImager. 

^cThe ratio is derived by dividing the value for an IJ with the value for an CJ. For example, 0.36 is the ratio of 0.89 (VλVκ) to 2.5 (VλJλ). 

^d—, both CJ and IJ values are too small to be significant for ratio calculation. 

^eThe quantitation is derived from Fig. 2B. Values are average intensities of the PCR products ± standard error on two different dilutions for each DNA sample (i.e., undiluted and threefold-diluted DNA). 

^fVλVκ. 

^gVλJκ.