TABLE 4.

Sequence analysis of Vλ1/2Jλ1 HJ made in s/+-ts and s/s-ts cells^a

Cloneb	Vλ1/2 coding sequence	N/Pc	Jλ1 RSS	No. of ntf deleted from germ line sequence for:
				Vλ1	Jλ1
Germ line	TACAGCAACCATTd		CACTGTG + 12 nt + GCAAAAAA
	TACAGCACCCATTTe
s/+-39
1-2 (2)	TACAGCAACCATT		CACTGTG + 12 nt + GCAAAAAA	0	0
1-3 (3)	TACAGCAACCA		CACTGTG + 12 nt + GCAAAAAA	2	0
1-5	TACAGCAACCA		CACTGTG + 12 nt + GCAAAAAA	2	0
2-1	TACAGCAACCA		CACTGTG + 12 nt + GCAAAAAA	2	0
2-2 (4)	TACAGCAACCATT		CACTGTG + 12 nt + GCAAAAAA	0	0
2-8 (2)	TACAGCACCCATTT	G	CACTGTG + 12 nt + GCAAAAAA	0	0
s/s-39
3-6	TAC		GCAAAAAA	10	19
3-2 (2)			3 nt + GCAAAAAA	24	4
3-3 (5)			12 nt + GCAAAAAA	40	7
s/s-39-33
4-5			8 nt + GCAAAAAA	48	11
4-6 (4)				31	37
5-3 (3)			CACTGTG + 12 nt + GCAAAAAA	34	0
5-9			CACTGTG + 12 nt + GCAAAAAA	82	0
5-2 (3)				18	26
5-6				32	26
5-4 (2)				67	30
5-14				37	34
5-8				33	38
5-5 (6)				33	50

^aPCR products, amplified from s/+ or s/s cells cultured at either 39°C for 3 days (s/+-39 and s/s-39) or 39°C for 3 days followed by 2 days at 33°C (s/s-39-33), were cloned and sequenced. 

^bSome sequences are compiled from two different experiments. The number before the hyphen designates the individual PCR, and the number after the hyphen represents individual repeats of cloning. The number in parentheses indicates the number of clones for the particular sequence. 

^cN and P nucleotide addition. 

^dVλ1. 

^eVλ2. 

^fnt, nucleotides.