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. 2021 Nov 27;21:629. doi: 10.1186/s12935-021-02339-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effect of let-7a mimic and dox on mitochondrial and cellular parameters in MDA-MB-231 and MCF-7 cells. RT-qPCR was used to determine mtDNA level in MDA-MB-231 and MCF-7 cells. mtDNA/nDNA ratio represent mtDNA number in A MDA-MB-231, and B MCF-7 cells. ND4 used for mitochondrial gene was normalised to nuclear β-Actin. Effect of dox and Let-7a on cellular ATP levels in C MDA-MB-231 and D MCF-7 cells. E Flow cytometric analysis of mitotracker green in MCF-7 cells. F and G MCF-7 cells were transfected with mimic/antisense of let-7a for 24 h. Western blot analysis showing expression levels of mitochondrial biogenesis markers TFAM (28KDa) and PGC-1α (100KDa). The data are represented as mean + SD, analyzed by unpaired t-test, n = 3. (p < 0.05). (**p < 0.01), (***p < 0.001), (****p < 0.0001)