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. 2021 Nov 27;21:629. doi: 10.1186/s12935-021-02339-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Measurement of oxygen concentration using Oroboros O2K Respirometer. Representative graphs showing measurement of oxygen consumption rate upon addition of different substrates and inhibitor in A MDA-MB-231 cells and B MCF-7 cells. C Measurement of oxygen concentration after combined treatment of 2-DG and let-7a in MDA-MB-231 cells. Bar graphs represents oxygen concentration in ρ⁰ cells of D MDA-MB-231 and E MCF-7 cells. Measurement of mitochondrial complex I activity in let-7a transfected (mimic/anti) MDA-MB-231 and MCF-7 cells. Data is represented as absorbance versus time. F Showing Complex I activity in MDA-MB-231cells and G MCF-7 cells. H Measurement of lactate levels in MDA-MB-231 and MCF-7 cells. Let-7a showing inhibition of glycolysis in MDA-MB-231 cells. Effect of let-7a on adipogenesis. I Graphs representing staining of Oil Red O in MCF-7 cells transfected with mimic/antisense of let-7a. Level of significance was tested by Two-way anova in Fig A, B and C, (****p < 0.0001). The data are represented as mean + SD analyzed by unpaired t-test, n = 3. (p < 0.05). (**p < 0.01)