Table 2.

Genes and primers for pyrosequencing

Gene	Genomic position of CpG sites (GCRh37/hg19)	Primer sequence
ABCA1	chr9:107,690,762; chr9:107,690,770 (cg14019050); chr9:107,690,773; chr9:107,690,791; chr9:107,690,797	5′-AACAAATTCCACTAATACCCTTAACT-3′ 5′-biotin-GGGTGGAGGGTATAGTAGGT-3′ Seq 5′-AACAAATTCCACTAATACCCTTAACT-3′
ABCG1 locus 1	chr21:43,642,336; chr21:43,642,354; chr21:43,642,367 (cg27243685)	5′-TGAGTTTAGGAGGTTAAGGAGAAATT-3′ 5′-biotin-CAAATAAACCAACAACAAAACAATAC-3′ Seq 5′-TGAGTTTAGGAGGTTAAGGA-3′
ABCG1 locus 2	chr21:43,656,587 (cg06500161); chr21:43,656,590	5′-GTAAGGTTTGGGGTTATTTTAGTGG-3′ 5′-biotin-AAAACAAACCCTTAAATCTCTTTACAT-3′ Seq 5′-GAGATTAGGGTTTTTTTTAGATA-3′