Fig. 9.

Effect of CT-133 on mRNA expression of pro-inflammatory cytokines (TNF-α, IL-β, IL-6) and a chemokine (KC) in LPS- and PGD₂-stimulated primary macrophages. Primary macrophages were pretreated with CT-133 for 1 h and further treated with CT-133 and LPS/PGD₂ for 24 h, and then total RNA was extracted for analysis of expression of IL-β (A and E), TNF-α (B and F), IL-6 (C and G) and KC (D and H) using RT-PCR. ^##P < 0.01 versus vehicle + vehicle group; *P < 0.05, **P < 0.01 versus LPS/PGD2 + vehicle group. Values are expressed as mean ± S.E.M., n = 3 for each group.