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. 2021 Nov 23;15:4741–4757. doi: 10.2147/DDDT.S334421

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© 2021 Wu et al.

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Saikosaponin D reduced the expression level of osteoclast-related genes induced by RANKL in vitro. (A) mRNA expression profile of OMG (osteoclast marker genes) (time-dependent). RT-qPCR (real-time quantitative PCR) was performed on total RNA extracted from cells stimulated by RANKL (50ng/mL) and treated with 2μM SSD for the indicated time points during the 5 day osteoclast culture. With β-actin as the reference, RANKL-induced related mRNA expressions of Dcstamp, MMP9, NFATc1, c-FOS, Atp6V0d2, and Acp5 were normalized. (B) mRNA expression profile of OMG (dose-dependent). RT-qPCR was performed on total RNA extracted from cells stimulated by RANKL (50ng/mL) and treated with various concentrations of SSD for 5 days. With β-actin as the reference, RANKL-induced related mRNA expressions of Dcstamp, MMP9, NFATc1, c-FOS, V-ATPase-d2, and Acp 5 were normalized. Mean ± SD is used to describe the data. Above experiments were conducted independently at least 3 times. ****P <0.0001, ***P<0.001, **P <0.01, *P <0.05.