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. Author manuscript; available in PMC: 2021 Nov 27.
Published in final edited form as: Nat Biotechnol. 2021 May 3;39(10):1220–1227. doi: 10.1038/s41587-021-00900-z

Fig. 3 |. Multiplex panel for the detection of cancer driver gene mutations.

Fig. 3 |

a, Recovery and coverage of the 48 amplicons within the multiplex panel. The horizontal axis displays the position downstream of the 3′ end of the second gene-specific primer (GSP2). The gradual decline in coverage with increasing distance from the 3′ primer end is a consequence of the input DNA fragmentation pattern. Details regarding the theoretical recovery of reads with specific amplicon lengths are discussed in the Supplementary Note. b, Duplex recoveries for each of the 48 amplicons with varying levels of sequencing depth. Recoveries were invariant beyond a threshold level of sequencing, suggesting that they are not artificially inflated due to polymerase or sequencing errors in the UID sequences.