Table 1.
Concentrations of total and MTB-derived urine cfDNA detected after Q Sepharose extractiona
| Median (IQR) | Range | ||
|---|---|---|---|
| Total cfDNA concentrationb | Eluate (ng/µl) | 5.1 (2.7–11.9) | 1.1–85.6 |
| Urine (ng/ml) | 25.5 (13.3–59.3) | 5.4–428 | |
| Estimated MTB cfDNA concentrationc | Eluate (copies/µl) | 5.2 (0.8–6.5) | 0.1–792 |
| Urine (copies/ml) | 26 (4.0–32.4) | 0.6–3958 |
cfDNA, cell-free DNA; IQR, interquartile range; MTB, Mycobacterium tuberculosis.
a
The detected concentrations of total and MTB-specific cfDNA in each sample selected for sequencing are given in Supplementary Material Table S1.
b
Measured using the Qubit HS dsDNA kit.
c
Measured by 40 bp qPCR targeting the variable copy number insertion sequence IS6110.