Figure 3.
Concentration-dependent block by CPP of the EPSPNMDA in vitro. A) Evoked field potentials were measured in the CA1 area of hippocampal brain slices, in the presence pharmacological blockers of AMPA (10 μM CNQX) and GABAA receptors (10 μM bicuculline), the NMDAR co-agonist glycine (3μM), and a low concentration of Mg++ (0.1 mM). Calibration bars 0.3 mV (300 nM) or 0.5 mV (100 nM, 1 μM), 50 mS. B) Time series plot of evoked responses during wash-in and washout of CPP. C) Summary of CPP block of at EPSPNMDA (filled circles, mean ± sem) at concentrations from 100 nM – 10 μM. n=2-5 per concentration. The shaded vertical bar shows the estimated CPP concentration corresponding to IC50 for fear conditioning to context in vivo. The open circles show relative block of long-term potentiation, for comparison (see Fig. 5 for details).