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. Author manuscript; available in PMC: 2022 Apr 14.
Published in final edited form as: Oncogene. 2021 Oct 14;40(47):6527–6539. doi: 10.1038/s41388-021-02053-4

Figure 1: Design and characterization of a library of MYC-targeting antisense oligonucleotides.

Figure 1:

(a) Schematic of the MYCASO library with approximate seed site locations of each of 23 oligonucleotides along the MYC transcript. Arrow represents the major translational start site. (b) Sequences of MYCASOs and NT-ASO used as control. Locations of LNA bases are highlighted in red. (C) Immunoblotting for MYC and alpha-tubulin in HeLa cells. Top panel shows whole cell extracts from HeLa cells treated with 10 nM MYCASO formulated in Lipofectamine 2000 (Lipo) for 24 hours. Bottom panel shows HeLa cells with 10 μM gymnotic-treated MYCASO for 72 hours.