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. 2021 Nov 27;7:368. doi: 10.1038/s41420-021-00756-x

Fig. 3. Targeting MALAT1 dampened inflammation and NLRP3 inflammasome-induced pyroptosis in M1 macrophages.

Fig. 3

Isolated KCs were transfected with siNC or siMALAT1 and treated with PBS (control), LPS, or IFN-γ. A F4/80 expression in indicated cells was examined by immunofluorescence (green). Cell nuclei were stained with DAPI (blue). Expressions of F4/80 and Ly6c proteins were detected by western blot (B), of MALAT1, F4/80, Ly6c, IL-6, iNOS, TNF-α, and MCP-1 by RT-qPCR (C), and of pyroptosis-related markers, NLRP3, pro-caspase-1, Caspase-1, and GSDMD-N by western blot (D) from indicated cells. E The productions of IL-1β and IL-18 in supernatants of indicated cells were measured by ELISA. *P < 0.05, **P < 0.01, ***P < 0.001.