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. 2021 Nov 27;7:368. doi: 10.1038/s41420-021-00756-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A The protein levels of USP8 and TAK1 were examined by western blot in KCs overexpressing control (NC) or USP8. B The interaction between USP8 and TAK1 was examined by Co-IP in HEK293T cells co-transfected with Myc-tagged TAK1 and Flag-tagged USP8 (left and middle panels). C The interaction between endogenous USP8 and Myc-TAK1, and that between endogenous TAK1 and Flag-USP8 was examined by Co-IP in HEK293T cells. D KCs were transfected with Myc-TAK1 and treated with or without MG132. The level of TAK1 was examined by western blot. E KCs cells from D were treated with cycloheximide (CHX) for indicated time periods. The expression of TAK1 was examined by western blot. F si-NC- or si-USP8-transfected KCs were treated with CHX for indicated time periods. The protein levels of TAK1 and USP8 were examined by western blot.