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. 2021 Nov 27;12(12):1109. doi: 10.1038/s41419-021-04100-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Structure and mechanism of action of HER2 CAR-T. B Surface expression of the HER2-CAR on lentivirus infected T cells was detected by flow cytometry on 10th day after lentivirus infection. C Procedure of allogeneic HER2 CAR-T therapy. D, E Cytotoxicity assays and cytokine release assays (*P < 0.05, **P < 0. 01, ***P < 0. 001, compared with NT-T of each sub-group, two-tailed t-test; ^#P < 0.05, compared with 1:1 of each sub-group, Kruskal–Wallis test followed by the Dunn’s multiple comparison test). F An apoptosis kit was conducted in the co-culture model at the E/T ratio of 10:1, and the representative images of cell apoptosis are shown (***P < 0.001, compared with NT-T, two-tailed t-test).