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. 2021 Nov 27;12(12):1109. doi: 10.1038/s41419-021-04100-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A 7 days after lentivirus infection, an apoptosis detection kit was used to detect the apoptosis of T cells, and the representative images are shown. B The difference in the expression levels of three exhaustion markers between HER2 CAR-T and NT-T group (HER2 CAR-T (%) - NT-T (%)) as detected by flow cytometry on 7 days after lentivirus infection. C 14 days after lentivirus infection, karyotype analysis was performed to detect chromosomal alterations in CAR-T cells. D, E H&E was performed to evaluate the pathological changes in Heart, Liver, Lung, and Kidney, and the representative images are shown. The scale bar is marked in each image. F, G IHC assay was performed to observe F4/80 or CD 68 positive macrophages in murine lung tissues, and the representative images are shown. The scale bar is marked in each image.