Fig. 4.

Basal accumulation of CpaGFP puncta depends on dia. (A) Live wild-type and dia embryos during interphase 13 expressing CpaGFP. The top panels display a z-section of the actin caps (1 µm), whereas the bottom panels show a z-section of the intercap region (5 µm), as indicated in the schematic representation, during early (2 min) and late (8 min) interphase. (B) Apical (cap) to basal (intercap) distribution of CpaGFP clusters within the field of view of four imaged embryos per genotype as indicated. The number of clusters was normalized to the first apical slice. Solid lines represent early interphase (2 min), dashed lines late interphase (8 min) in wild-type (blue) and dia (red) embryos. (C) To obtain maximum intensity projections shown in D, z-stacks of frontal sections were projected as orthogonal views. (D) Orthogonal views of wild-type (left) and dia (right) embryos expressing CpaGFP during interphase 13 at indicated time points. The positions of nuclei are indicated by dashed circles. Scale bars: 10 µm.