FIG. 5.

Respiratory deficiency of MB43-nam9-1 is suppressed by the absence of or by overproduction of Hsp104. The respective derivatives of MB43 (compare to Table 1) were transferred to YPGly plates and incubated for 3 days at 30°C. In order to determine the content of Hsp104 and Nam9 or Nam9-1p, the strains were grown on low-glucose medium and harvested at the point of glucose exhaustion (see Materials and Methods). Total protein was extracted by alkaline lysis and analyzed by Western blotting using antibodies specific for Hsp104 and Nam9p.