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Respiratory deficiency of MB43-nam9-1 can be overcome by expression of the C-terminal portion of Sup35. MB43-nam9-1 derivatives were transferred to YPGly plates and incubated for 3 days at 30°C. MB43-nam9-1 was transformed with empty control vector ([−]), pEMBLyex4-3ATG ([SUP35-C]), pEMBLyex4-SUP35 ([SUP35]), and pA1/4 ([HSP104↑]) (Table 1; see also Materials and Methods).
