Table 1.
Transposon-insertion sequencing (Tn-Seq) statistics
|
Replicate |
Sequencing yield |
No. of reads after normalization* |
No. of Tn-end containing reads |
Replicon |
Read count (unique TA sites) |
TA hits† |
Insertion density† |
Mean read count over non-zero TA‡ |
|---|---|---|---|---|---|---|---|---|
|
1 |
23,662,661 |
23,179,825 |
20, 267, 199 |
Chromosome |
20,168,343 |
60,186 |
70.5 |
335.1 |
|
pLM16734 |
938, 617 |
1,197 |
88.0 |
784.1 |
||||
|
2 |
23,179, 825 |
23,179, 825 |
20, 309, 279 |
Chromosome |
20, 209, 37 |
60,715 |
71.2 |
332.9 |
|
pLM16734 |
92, 4 781 |
1,218 |
89.5 |
759.3 |
||||
|
|
|
|
|
|
|
|
|
|
*Numbers of reads were normalized based on the lowest sequencing yield (i.e. replicate 2).
†The complete X. hortorum pv. vitians LM16734 genome contains 85 314 TA sites in the chromosome and 1361 in plasmid pLM16734. Insertion density was calculated as the ratio of TA sites with at least one read mapped over the total number of TA sites in the replicon.
‡Mean value of mapped reads per TA site with at least one read.