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. Author manuscript; available in PMC: 2021 Nov 28.
Published in final edited form as: Anal Biochem. 2013 May 24;440(1):96–107. doi: 10.1016/j.ab.2013.05.012

Figure 5. Simulated binding isotherms showing how curve shape varies with KD.

Figure 5

Binding data simulated using the quadratic binding equation with the stoichiometric ratio fixed at 1 arbitrary molar unit. (A) The concentration of receptor was fixed at 1 unit, and the concentration of ligand varied from 0.01 to 100 units. (B) The concentration of ligand was fixed at 1 unit, and the concentration of receptor varied from 0.01 to 100 units. For each isotherm KD was fixed at 0.001 (blue line), 0.01 (red line), 0.1 (green line), or 1 (orange line) units respectively.