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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Nat Neurosci. 2021 Apr 19;24(6):886–896. doi: 10.1038/s41593-021-00841-5

Fig. 2 |. Simultaneous multimodal recordings from the hippocampus and cortex.

Fig. 2 |

a, Surgical setup. Neuro-FITM was first inserted into the hippocampus (left) and then the shank was bent down to the right side to allow lowering the microscope objective and clearing the field of view for imaging (right).

b, Penetrating trajectory of Neuro-FITM in the hippocampus visualized by immunostaining against glia acidic protein (GFAP). Arrowhead: trajectory in CA1 pyramidal layer.

c, Field of view of wide-field calcium imaging during experiment. Note that the array shank was largely invisible and generated minimal shadows on the overlaying cortex.

d, Representative LFP recordings from the channels of the Neuro-FIRM probe in one recording session. Multiple channels adjacent (red) to the pyramidal layer of CA1 detected SWRs.

e, Examples of simultaneously recorded hippocampal SWRs (left column) and cortical activity (right column, single image frames at SWR onset). Cortical activity shows diverse spatial patterns during SWRs.