Figure 6. Iopromide induces the formation of acidic vacuoles in the autophagosomes. HEK 293 cells were incubated with 50 mgI/ml iopromide for 48 h. The cells were stained with acridine orange (AO). (A) Representative fluorescent images of AO-stained cells. (B) The relative fluorescence intensity is expressed relative to that of the untreated control. (C) Representative histograms AO- and acidic vesicular organelles (AVOs)-stained cells generated using the image cytometer analysis. The results are expressed relative to those of the untreated control and values are presented as mean±SE (n=3) (***p<0.001).
