Figure 9. Iopromide elicits reactive oxygen species (ROS) production. HEK 293 cells were incubated with 50 mgI/ml iopromide for 24 h. The cells were stained with 50 μM CM-H₂DCFDA (General Oxidative Stress Indicator). (A) Representative cytometer histogram of CM-H₂DCFDA stained cells. (B) The percentage of ROS production in treated samples was compared with that of the untreated control. (C) The ROS scavenger (N-acetylL-cysteine; NAC) was added in iopromide-treated cells. Cell viability was evaluated using the MTT assay. The results are expressed relative to those of the untreated control and values are presented as mean±SE (n=3) (***p<0.001).