FIG. 2.
Construction of MET-regulated alleles of DHR1 and DHR2. (A) Growth rates of MET::HA-dhr1 (closed diamonds), MET::HA-dhr2 (closed squares), and wild-type (open squares) strains following addition of methionine to the medium. Values are corrected for dilution. (B) Western blot analysis of strains described in panel A. Total protein was extracted from similar amounts of cells (according to OD600), separated on SDS-polyacrylamide gels (8 and 15% PAGE for Dhr1p and Dhr2p, respectively), and transferred to nitrocellulose. Membranes were decorated with anti-HA antibodies. Two protein components of the signal recognition particle, Srp14p (16.4 kDa) and Srp68p (69.0 kDa), were decorated with specific antibodies as loading controls.