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. 2021 Nov 24;14:6175–6190. doi: 10.2147/JIR.S337031

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© 2021 Chen et al.

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Supplement of Lactobacillus reuteri reduced Th17 cells count and contributed to alleviation of psoriasis like symptoms. (A) Graphical representation of the experimental design. (B) Changes in animal’s weight of imiquimod treated mice colonized with saline (PS, n=7) or colonized with five different Lactobacillus species (n=6–8). (C) PASI score of PS, LA, LP, LR, LRH, LC and MIX measured at day 7. (D) Thickness of skin and ear measured by Digimatic Caliper at day 7. (E) Representative photographs of back skin and ear in PS (n=7), LA (n=6), LP (n=6), LR (n=8), LRH (n=6), LC (n=6) and MIX (n=6). Photos were taken at day 7. (F) H&E stained skin sections of PS, LA, LP, LR, LRH, LC and MIX mice after imiquimod treatment, with 3–5 mice for each group. (G) Quantification of histopathological score (0–2) after H&E staining of the ear and skin. (H and I) Treg and Th17 cells were analysed by flow cytometry for the expression of CD4⁺ CD25⁺ Foxp3⁺ and CD4⁺ IL-17A⁺, respectively. Percentages and counts of Th17 cells (H) and Treg cells (I) in the spleen were determined. (J) The ratio of Treg/Th17 cells. (K and L) Messenger RNA levels of key cytokines (K) and chemokines (L) in the skin, with 3–4 mice per each group. (M) The mRNA level of IL10 in the skin (n=3–4 per group). Data are presented as the mean ± SD: *p < 0.05, **p < 0.01.

Abbreviations: LA, Lactobacillus acidophilus; LP, Lactobacillus plantarum; LR, Lactobacillus reuteri; LRH, Lactobacillus rhamnosus; LC, Lactobacillus casei; MIX, the union of the five different species Lactobacillus.