Extended Data Fig. 8. Global analysis of FLN5 A3A3 and A3A3E6 chemical shift perturbations.

(a) Chemical shift perturbations of FLN5 A₃A₃ vs. FLN5 A₃A₃E₆ ($\mathrm{\Delta }\delta =\sqrt{\mathrm{\Delta }{\delta }_H^2+{\left(\mathrm{\Delta }{\delta }_N/5\right)}^2}$)). E₆ mutation sides are indicated using red bar charts. (b) T₂ relaxation measurements of isolated FLN5 A₃A₃ and FLN5 A₃A₃E_6. (c) Correlation plots of ¹H and ¹⁵N chemical shift perturbations in +26, +28, +31, +42 and +110 A₃A₃/A₃A₃E₆ RNCs for residues as indicated (283 K, 950 MHz). A global fit of $\mathrm{\Delta }\mathrm{\Delta }{G}_{Ubound-Ufree}^{A3A3-A3A3E6}$ is shown (red line) and red markers indicate the fitted values of Δδ_max (χ² = 70.17, dof = 66, χ²/ν = 1.06).

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