TABLE 4.
Increased levels of induced ectopic SSeCKS correlates with increased cytoplasmic cyclin D1 sequestrationa
| Cell type | TET concn (μg/ml) | % of cells showing staining of:
|
|
|---|---|---|---|
| Cytoplasm | Nucleus | ||
| S24/V | 0 | 3 ± 3 | 15 ± 4 |
| S24/V | 0.02 | 8 ± 4 | 34 ± 5 |
| S24/V | 0.5 | 7 ± 4 | 70 ± 4 |
| S24/D1 | 0 | 85 ± 6 | 32 ± 4 |
| S24/D1 | 0.02 | 29 ± 4 | 59 ± 4 |
| S24/D1 | 0.5 | 8 ± 6 | 96 ± 3 |
a
Cells grown on 22-mm2 coverslips in medium containing various concentrations of TET for 2 days were fixed and stained for cyclin D1 using PAb, as described in Materials and Methods. Independent fields of cells were counted (total of 250 to 300 cells for each analysis). Note that the decrease in nuclear staining in the S24/V cells correlating with decreasing TET concentration relates to the SSeCKS-induced downregulation of cyclin D1 (Fig. 8A).