Table 2.
Nucleotide sequences of primers (TLR-mediated signaling pathway-related cytokines, chemokines and negative regulators) for quantitative real-time PCR assay.
| Genes | Primer sequence (forward and reverse) | Accession number |
|---|---|---|
| TLR-2 | F: GGGGCTCACAGGCAAAATC | NM_001161650.1 |
| R: AGCAGGGTTCTCAGGTTCACA | ||
| TLR-4 | F:CCACTATTCGGTTGGTGGAC | NM_001030693.1 |
| R:ACAGCTTCTCAGCAGGCAAT | ||
| TNFSF15 | F- CCAAGAGCACACCTGACAGT | NM_001024578.1 |
| R- CACAGGTATCACCAGTGCGT | ||
| MyD88 | F:GGATGGTGGTCGTCATTTCA | NM_001030962.1 |
| R:GAGATTTTGCCAGTCTTGTCCA | ||
| TRAF-6 | F: CACAGAGGAGACGCAGGGATA | XM_001235884.1 |
| R: AACAGATCGGGCACTCGTATTT | ||
| NF-kB | F:TGGAGAAGGCTATGCAGCTT | NM_205134.1 |
| R:CATCCTGGACAGCAGTGAGA | ||
| IL-1β | F-CAGCAGCCTCAGCGAAGAG | NM_204524.1 |
| R-CTGTGGTGTGCTCAGAATCCA | ||
| IL-8 | F-GGCTTGCTAGGGGAAATGA | AJ009800 |
| R-AGCTGACTCTGACTAGGAAACTGT | ||
| IL-10 | F:CGCTGTCACCGCTTCTTCA | NM_001004414.2 |
| R:CGTCTCCTTGATCTGCTTGATG | ||
| IFN-γ | F-AAAGCCGCACATCAAACACA | NM_205149.1 |
| R-GCCATCAGGAAGGTTGTTTTTC | ||
| Tollip | F:CATGGTACCTGTGGCAATACC | NM_001006471 |
| R:GCACTGAGCGGATTACTTCC | ||
| PI3K | F:AACATCTGGCAAAACCAAGG | NM_001004410 |
| R:CTGCAATGCTCCCTTTAAGC | ||
| A20 | F:GAGAACGCAGAGCCTACACC | NM_001277522.1 |
| R:CCAACCTTCTTCCTGCACAT | ||
| SOCS-1 | F:GCTCTCAGGCTCGAGGTTAC | NM_001137648.1 |
| R:GCTTGCTCGAGTGATGCTACT | ||
| SOCS-6 | F: CAGATATCTTTGTGGACCAGGCAGTGAA | NM_001127312 |
| R: GGTAGCAAAGGTGAAAGTGGAGGGACATC |
Abbreviations: A20, protein A20; IFN-γ, interferon γ; IL, interleukin; MyD8, myeloid differential protein-88; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; PI3K, phosphatidylinositol 3-kinase; SOCS, suppressor of cytokine signaling; TLR, toll-like receptor; TNFSF15, tumor necrosis factor superfamily member 15; Tollip, toll-interacting protein; TRAF-6, TNF receptor-associated factor 6.